Modulation of Vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease
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چکیده
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Purification and characterization of a protease produced by Vibrio mimicus.
A protease produced by Vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on Sephacryl S-100 and Mono Q Monobeads. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. Conventional PAGE showed that the purified protease m...
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Vibrio mimicus is a Gram-negative bacterium, which causes gastroenteritis and is closely related to Vibrio cholerae. The environmental reservoir of this bacterium is far from defined. Acanthamoeba as well as Vibrio species are found in diverse aquatic environments. The present study was aimed to investigate the ability of A. castellanii to host V. mimicus, the role of bacterial protease on inte...
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Introduction: Esterases (E.C. 3.1.1.) are widely distributed in nature and catalyze the hydrolysis of ester bonds. Arylesterases (E.C. 3.1.1.2.) show a preferential substrate specificity for aromatic esters. The mammalian arylesterases play important roles in the detoxification of organophosphorous compounds and are used as diagnostic indicators for liver cirrhosis. Very little is known about t...
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An environmental study was done to examine the prevalence of Vibrio mimicus in some aquatic environments of Dhaka, Bangladesh, and of Okayama, Japan. Water samples from Dhaka environments and water and plankton samples from Okayama environments were quantitatively as well as qualitatively analyzed throughout the seasons for V. mimicus. The organism was isolated from Bangladesh environments thro...
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Thiazolidinediones are commonly used anti-diabetic drugs. Owing to the anti-inflammatory action of TZDs as a result of there action on the PPAR gamma receptor and a proposed action on the prostaglandins, these drugs can be tried in the acute exacerbations of COPD that are also commonly found among diabetic patients. An experimental study of one week was carried out at animal house of Army Medic...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2009
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2008.06827.x